علوم زیستی دریا
Mojtaba Alishahi; Mohammad MehdiI Haghparast Radmard; Maryam Shokoohmand; Farid Barati
Abstract
Regarding expansion of Litopenaeus vanamei culture in Iran, and the necessity to work on its reproduction system, conducting a research on its biological reproduction and sexual properties is of high value and sexual properties. Then in this study morphological properties of L. vanamei's sperm were evaluated ...
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Regarding expansion of Litopenaeus vanamei culture in Iran, and the necessity to work on its reproduction system, conducting a research on its biological reproduction and sexual properties is of high value and sexual properties. Then in this study morphological properties of L. vanamei's sperm were evaluated using phase contrast microscope, Atomic Force Microscopy technique and Electronic microscope. Wight of testis and spermotophore as well as density and survival rate of sperms were measured in L. vanamei. For this purpose, 20 mature male L. vanamei (35.37 ±4.3) were transferred from a local shrimp hatchery to laboratory. Biometrical indices as well as reproductive properties of shrimp (density, diameter and size of sperms, spermatophore weight and melanisation) were measured. Correlation coefficient between all indices were measured too. Results showed that testis and spermatophore weight were 0.18±o.01g and 0.08±0.01g respectively. Sperm count, survival rate and diameter of sperms were 41.19±3.83 per µl, %69.69±%2.39 and 39.36±10.8 respectively. The highest correlation coefficient was seen between shrimp weight and testis weight (R=0.958), sperm diameter (R=0.91) and sperm survival (R=0.716) respectively, although there was no significant correlation between abnormal sperm or melanized spermatophores and other biometrical indices.